Tryptone Sulfite Neomycin Agar Culture Medium
Item Name Code (INC) 68837
![]() |
Additional Information for Tryptone Sulfite Neomycin Agar Culture Medium
Tryptone Sulfite Neomycin Agar (TSN Agar) is a culture medium used in microbiology laboratories for the isolation and identification of Clostridium perfringens, a bacterium that can cause food poisoning and other infections. It is classified as an In Vitro Diagnostic Substance and falls under the supply class of Reagents, Test Kits, and Sets in the supply group of Medical, Dental, and Veterinary Equipment and Supplies.
TSN Agar contains tryptone, sulfite, neomycin, and agar. Tryptone is a source of nutrients for bacterial growth, sulfite acts as a selective agent to inhibit the growth of other bacteria, neomycin is an antibiotic that further inhibits the growth of unwanted bacteria, and agar provides a solid surface for bacterial colonies to grow on.
This culture medium is used to detect and enumerate Clostridium perfringens in various samples, such as food, water, and clinical specimens. It is particularly useful in food safety testing and epidemiological investigations.
To use TSN Agar, the medium is prepared according to the manufacturer's instructions, sterilized, and then poured into petri dishes or other suitable containers. The sample to be tested is then inoculated onto the agar surface and spread evenly. The plates are incubated at an appropriate temperature for the growth of Clostridium perfringens, typically around 37°C for 18-24 hours. After incubation, colonies of Clostridium perfringens appear as large, grayish-black colonies with a characteristic double zone of hemolysis.
The presence and enumeration of Clostridium perfringens on TSN Agar can help identify potential sources of contamination and aid in the prevention and control of foodborne illnesses.
Please note that the specific instructions for the preparation and use of TSN Agar may vary depending on the manufacturer and the intended application. It is important to refer to the product documentation and follow the recommended protocols for accurate and reliable results.
Related Item Names for INC 68837 Tryptone Sulfite Neomycin Agar Culture Medium
NATO Stock Numbers with Item Name 68837 Tryptone Sulfite Neomycin Agar Culture Medium Page 1 of 1
- 4138331210
- RNCC: 5 | RNVC: 2 | DAC: 3