Electrophoresis Blotter
Item Name Code (INC) 42784
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An item used to remove excess buffer or reagents during electrophoresis procedures such as cellulose acetate. May be absorbent paper.
Additional Information for Electrophoresis Blotter
Electrophoresis blotter is a type of laboratory equipment used in the field of molecular biology and biochemistry. It is specifically designed for transferring proteins or nucleic acids from a gel matrix to a solid support membrane, such as nitrocellulose or PVDF (polyvinylidene fluoride). This process is known as Western blotting or Southern blotting, depending on the type of molecule being transferred.
The electrophoresis blotter consists of a transfer tank, which holds the gel and the membrane, and a power supply that generates an electric field to facilitate the transfer of molecules. The transfer tank is equipped with a buffer reservoir and a sponge or filter paper stack that helps maintain a constant buffer flow and ensures efficient transfer.
The blotting process involves placing the gel and the membrane in contact with each other, sandwiched between layers of filter paper or sponges soaked in transfer buffer. The electric field is then applied, causing the proteins or nucleic acids to migrate out of the gel and onto the membrane. This allows for further analysis, such as protein detection using specific antibodies or DNA hybridization.
Electrophoresis blotters are available in various sizes and configurations to accommodate different gel sizes and transfer requirements. They are an essential tool in molecular biology and biochemistry research, enabling the study of protein expression, DNA sequencing, and genetic analysis.
In summary, electrophoresis blotters are laboratory equipment used for transferring proteins or nucleic acids from a gel matrix to a solid support membrane for further analysis in molecular biology and biochemistry research.
Related Item Names for INC 42784 Electrophoresis Blotter
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