Desoxycholate Citrate Agar Culture Medium
Item Name Code (INC) 40610
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Additional Information for Desoxycholate Citrate Agar Culture Medium
Desoxycholate Citrate Agar (DCA) is a culture medium used in microbiology laboratories for the isolation and differentiation of enteric bacteria, particularly members of the Enterobacteriaceae family. It is classified as an in vitro diagnostic substance and falls under the supply class of In Vitro Diagnostic Substances, Reagents, Test Kits, and Sets in the supply group of Medical, Dental, and Veterinary Equipment and Supplies.
DCA contains various ingredients that promote the growth of enteric bacteria while inhibiting the growth of other microorganisms. The main components of DCA include:
1. Desoxycholate: This bile salt inhibits the growth of Gram-positive bacteria and some Gram-negative bacteria, allowing for the selective isolation of enteric bacteria.
2. Sodium Citrate: This compound acts as a pH buffer and enhances the recovery of enteric pathogens.
3. Lactose: This carbohydrate serves as a fermentable substrate for the differentiation of lactose-fermenting and non-lactose-fermenting bacteria.
4. Neutral Red: This pH indicator helps in the differentiation of lactose-fermenting colonies, which appear red, from non-lactose-fermenting colonies, which appear colorless.
5. Agar: This solidifying agent provides a solid surface for bacterial growth and colony formation.
To use DCA, a sample containing enteric bacteria is streaked onto the surface of the agar plate and incubated at an appropriate temperature. After incubation, the colonies can be examined for their morphology, color, and other characteristics to identify different species of enteric bacteria.
It is important to note that DCA is intended for in vitro diagnostic use only and should be handled and disposed of according to proper laboratory protocols and regulations.
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Related Item Names for INC 40610 Desoxycholate Citrate Agar Culture Medium
NATO Stock Numbers with Item Name 40610 Desoxycholate Citrate Agar Culture Medium Page 1 of 1
- 8031
- RNCC: 3 | RNVC: 2 | DAC: 4
- AX021113
- RNCC: 3 | RNVC: 2 | DAC: 3