Brilliant Green Bile Agar Culture Medium
Item Name Code (INC) 40603
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Additional Information for Brilliant Green Bile Agar Culture Medium
Brilliant Green Bile Agar (BGBA) is a culture medium used in microbiology laboratories for the isolation and differentiation of enteric bacteria, particularly Salmonella and Shigella species. It belongs to the supply class of In Vitro Diagnostic Substances, Reagents, Test Kits, and Sets, which are substances and materials used for diagnostic purposes in laboratory settings.
BGBA is a selective and differential medium that contains various components to inhibit the growth of non-enteric bacteria and allow the growth of enteric pathogens. The main components of BGBA include:
1. Peptone: Provides a source of nitrogen and other essential nutrients for bacterial growth.
2. Bile salts: Inhibit the growth of Gram-positive bacteria and some Gram-negative bacteria, allowing for the selective growth of enteric pathogens.
3. Brilliant Green dye: Inhibits the growth of Gram-positive bacteria and some Gram-negative bacteria, while allowing the growth of Salmonella and Shigella species.
4. Lactose: A carbohydrate source that allows for the differentiation of lactose-fermenting and non-lactose-fermenting bacteria.
5. Neutral red: A pH indicator that helps differentiate lactose-fermenting colonies (pink/red) from non-lactose-fermenting colonies (colorless).
To use BGBA, a sample suspected of containing enteric pathogens is streaked onto the surface of the agar plate. After incubation, the presence of characteristic colonies can be observed. Salmonella species typically produce colorless colonies, while Shigella species produce green colonies. Other non-enteric bacteria are inhibited by the selective components of the medium.
Overall, Brilliant Green Bile Agar Culture Medium is an important tool in the laboratory diagnosis of enteric bacterial infections, particularly Salmonella and Shigella species. It is classified under the supply group of Medical, Dental, and Veterinary Equipment and Supplies, as it is used in medical and veterinary diagnostic settings.
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