Macconkey Agar Culture Medium
Item Name Code (INC) 37708
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Additional Information for Macconkey Agar Culture Medium
MacConkey Agar is a selective and differential culture medium used in microbiology laboratories. It is primarily used for the isolation and differentiation of gram-negative bacteria, particularly members of the Enterobacteriaceae family.
MacConkey Agar contains various ingredients that make it selective and differential. The medium contains bile salts and crystal violet, which inhibit the growth of gram-positive bacteria and allow the growth of gram-negative bacteria. It also contains lactose as a fermentable carbohydrate and neutral red as a pH indicator.
When lactose-fermenting bacteria grow on MacConkey Agar, they produce acid, which lowers the pH of the medium. This causes the neutral red indicator to turn pink or red, indicating lactose fermentation. Non-lactose fermenters do not produce acid and appear colorless on the medium.
MacConkey Agar is commonly used in clinical laboratories to isolate and identify enteric pathogens, such as Escherichia coli, Salmonella, and Shigella species. It is also used to differentiate lactose-fermenting and non-lactose fermenting bacteria, which can be helpful in identifying certain bacterial species.
In summary, MacConkey Agar is a culture medium used for the selective isolation and differentiation of gram-negative bacteria, particularly enteric pathogens. It is an important tool in microbiology laboratories for diagnosing and studying bacterial infections.
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