Mannitol Salt Agar Culture Medium
Item Name Code (INC) 37081
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Additional Information for Mannitol Salt Agar Culture Medium
Mannitol Salt Agar (MSA) is a culture medium used in microbiology to isolate and differentiate certain types of bacteria, particularly Staphylococcus species. It is classified as an In Vitro Diagnostic Substance and falls under the supply class of Reagents, Test Kits, and Sets in the supply group of Medical, Dental, and Veterinary Equipment and Supplies.
MSA contains the following components:
1. Mannitol: It is a type of sugar that serves as the fermentable carbohydrate in the medium. Some bacteria can utilize mannitol for growth and produce acid as a byproduct.
2. Salt: MSA contains a high concentration of salt, usually sodium chloride. This high salt concentration inhibits the growth of most bacteria, except for certain halophilic (salt-loving) organisms like Staphylococcus species.
3. Phenol Red: It is a pH indicator that turns yellow when acid is produced due to mannitol fermentation. This helps in differentiating between mannitol-fermenting and non-fermenting bacteria.
The purpose of using MSA is to selectively isolate and identify Staphylococcus species, particularly Staphylococcus aureus. Staphylococcus aureus is capable of fermenting mannitol, which leads to the production of acid and a change in the color of the medium from red to yellow. Other non-fermenting bacteria will not cause a color change.
By observing the growth and color change on MSA, microbiologists can differentiate between different types of Staphylococcus species and identify potential pathogens. This information is crucial for diagnosing and treating infections caused by Staphylococcus bacteria.
Please note that the use of MSA should be performed by trained professionals in a laboratory setting following appropriate safety protocols.
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