Hektoen Enteric Agar Culture Medium
Item Name Code (INC) 36632
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Additional Information for Hektoen Enteric Agar Culture Medium
Hektoen Enteric Agar (HEA) is a culture medium used in microbiology laboratories for the isolation and differentiation of enteric bacteria, particularly those belonging to the family Enterobacteriaceae. It is classified as an in vitro diagnostic substance and falls under the supply class of In Vitro Diagnostic Substances, Reagents, Test Kits, and Sets in the supply group of Medical, Dental, and Veterinary Equipment and Supplies.
HEA contains a combination of selective and differential ingredients that allow for the growth of enteric bacteria while inhibiting the growth of other organisms. The medium contains bile salts and dyes that inhibit the growth of Gram-positive bacteria and most Gram-negative bacteria, except for enteric bacteria. It also contains lactose, sucrose, and salicin as fermentable carbohydrates, along with indicators such as bromothymol blue and acid fuchsin.
When enteric bacteria grow on HEA, they produce characteristic colonies with different colors based on their ability to ferment the carbohydrates present in the medium. For example, lactose-fermenting bacteria produce colonies that appear yellow or orange, while non-lactose fermenters produce colonies that are green or blue-green. This differentiation helps in the identification of specific enteric bacteria, such as Salmonella and Shigella species.
HEA is commonly used in clinical laboratories for the isolation and identification of enteric pathogens from clinical specimens, such as stool samples. It is an essential tool in diagnosing gastrointestinal infections and plays a crucial role in public health surveillance and outbreak investigations.
Please note that the information provided is a general overview, and specific protocols and guidelines may vary depending on the laboratory and the intended use of the medium. It is always recommended to consult the manufacturer's instructions and relevant scientific literature for detailed information on the preparation and use of Hektoen Enteric Agar Culture Medium.
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