Additional Information for Dithiothreitol
Dithiothreitol (DTT) is a commonly used reducing agent in biochemistry and molecular biology. It is a small molecule with the chemical formula C4H10O2S2. DTT is a white crystalline powder that is soluble in water.
DTT is primarily used to reduce disulfide bonds in proteins and peptides. Disulfide bonds are covalent bonds between two sulfur atoms, and they play a crucial role in stabilizing the three-dimensional structure of proteins. By breaking these bonds, DTT helps to denature proteins and peptides, making them more accessible for further analysis or manipulation.
In addition to its reducing properties, DTT also acts as an antioxidant, protecting biomolecules from oxidative damage. It can scavenge reactive oxygen species and free radicals, preventing them from causing cellular damage.
DTT is commonly used in various laboratory techniques, such as protein purification, protein electrophoresis, and protein structure determination. It is often included in buffers and reaction mixtures to maintain a reducing environment and prevent the formation of disulfide bonds.
When working with DTT, it is important to handle it with care as it can be toxic if ingested or inhaled. It is recommended to wear appropriate personal protective equipment, such as gloves and a lab coat, and to work in a well-ventilated area.
Overall, DTT is a valuable tool in biochemical research, allowing scientists to manipulate and study proteins and peptides by reducing disulfide bonds and maintaining a reducing environment.
Related Item Names for INC 32511 Dithiothreitol
NATO Stock Numbers with Item Name 32511 Dithiothreitol Page 1 of 1
- D0632
- RNCC: 5 | RNVC: 1 | DAC: 4
- D9779
- RNCC: 3 | RNVC: 2 | DAC: 3
- 0281-5G
- RNCC: 3 | RNVC: 2 | DAC: 4
- A2452-5GR
- RNCC: 3 | RNVC: 2 | DAC: 2