Kligler Iron Agar Culture Medium
Item Name Code (INC) 29745
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Additional Information for Kligler Iron Agar Culture Medium
Kligler Iron Agar (KIA) is a type of culture medium used in microbiology laboratories for the identification and differentiation of bacteria, particularly those belonging to the Enterobacteriaceae family. It falls under the supply class of In Vitro Diagnostic Substances, Reagents, Test Kits, and Sets in the supply group of Medical, Dental, and Veterinary Equipment and Supplies.
Kligler Iron Agar contains several components that allow for the detection of specific metabolic activities of bacteria. The medium consists of peptones, beef extract, yeast extract, glucose, lactose, sucrose, ferrous sulfate, and phenol red indicator.
The KIA medium is primarily used to differentiate bacteria based on their ability to ferment sugars and produce hydrogen sulfide gas. The presence of glucose, lactose, and sucrose in the medium allows for the detection of sugar fermentation. If a bacterium can ferment any of these sugars, it produces acid, which lowers the pH of the medium and causes a color change from red to yellow.
Additionally, KIA medium contains ferrous sulfate, which acts as a source of iron. Bacteria that can reduce ferrous sulfate to ferrous sulfide produce hydrogen sulfide gas, resulting in the formation of a black precipitate.
By observing the color changes and the presence or absence of gas and black precipitate, microbiologists can identify and differentiate bacteria based on their metabolic characteristics.
Overall, Kligler Iron Agar Culture Medium is an important tool in microbiology laboratories for the identification and differentiation of bacteria, particularly those belonging to the Enterobacteriaceae family.
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