Lowenstein-Jensen Culture Medium
Item Name Code (INC) 10420
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Additional Information for Lowenstein-Jensen Culture Medium
Lowenstein-Jensen Culture Medium is a type of culture medium used in microbiology laboratories for the isolation and cultivation of Mycobacterium species, particularly Mycobacterium tuberculosis. It is classified under the supply class In Vitro Diagnostic Substances, Reagents, Test Kits And Sets in the supply group Medical, Dental, And Veterinary Equipment And Supplies.
The Lowenstein-Jensen Culture Medium is a solid medium that contains various nutrients and selective agents to support the growth of Mycobacterium species while inhibiting the growth of other bacteria and fungi. It is named after its developers, Lowenstein and Jensen, who first described the medium in the early 1900s.
This culture medium is commonly used in the diagnosis of tuberculosis (TB) as it allows for the growth and identification of Mycobacterium tuberculosis, the causative agent of TB. It is also used for drug susceptibility testing to determine the effectiveness of different antibiotics against the isolated Mycobacterium strains.
The Lowenstein-Jensen Culture Medium is typically prepared as a solid slant in a test tube. It contains ingredients such as egg or serum, glycerol, malachite green, and various salts and nutrients. The medium is heated and sterilized before use to ensure the absence of any contaminants.
To use the Lowenstein-Jensen Culture Medium, a clinical specimen suspected of containing Mycobacterium tuberculosis is inoculated onto the surface of the medium. The test tube is then incubated at a specific temperature (usually 37°C) for several weeks to allow the slow-growing Mycobacterium species to grow and form visible colonies.
The colonies that appear on the Lowenstein-Jensen Culture Medium can be further analyzed using various biochemical tests and staining techniques to confirm the presence of Mycobacterium tuberculosis and to determine its drug susceptibility profile.
Overall, the Lowenstein-Jensen Culture Medium is an essential tool in the laboratory diagnosis of tuberculosis and plays a crucial role in the identification and management of this infectious disease.
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